TIMP2 antibody

Catalog No. ABIN214221

The Mouse Monoclonal anti-TIMP2 antibody (Clone F27P3A4) (ABIN214221) specifically detects TIMP2 in WB, IHC, ELISA and IHC (p). The antibody is reactive with Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Dog, Horse, Pig, Monkey, Sheep, Chicken and Hamster samples.

Quick Overview for TIMP2 antibody (ABIN214221)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Dog, Horse, Pig, Monkey, Sheep, Chicken, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: F27P3A4

Product Details anti-TIMP2 Antibody

Specificity: Ovalbumin-conjugated synthetic Peptide DSGNDIYGNPI

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Sheep, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Seabass, Zebrafish (100%) Xenopus, Medaka, Pufferfish (91%) Bat, Stickleback (82%).

Purification: Affinity purified

Immunogen: Synthetic peptide from human TIMP2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Sheep, Bovine, Dog, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Opossum, Guinea pig, Turkey, Chicken, Lizard, Seabass, Zebrafish (100%), Xenopus, Medaka, Pufferfish (91%), Bat, Stickleback (82%).

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background: Name/Gene ID: TIMP2

Synonyms: TIMP2, Metalloproteinase inhibitor 2, TIMP-2, CSC-21K

Gene ID: 7077

UniProt: P16035

Pathways: cAMP Metabolic Process