RPA3 antibody

Catalog No. ABIN214259

The Mouse Monoclonal anti-RPA3 antibody has been validated for WB, IHC, IP and IHC (p). It is suitable to detect RPA3 in samples from Human and Mouse.

Quick Overview for RPA3 antibody (ABIN214259)

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RPA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 111

Product Details anti-RPA3 Antibody

Specificity: Full-length human RPA-14 expressed in E. coli.

Purification: Protein G purified

Immunogen: Recombinant human RPA3.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. Sourced in TCS.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RPA3

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Alternative Name: RPA3

Background: Name/Gene ID: RPA3

Synonyms: RPA3, REPA3, Replication factor A protein 3, Replication protein A3 (14kD), Replication protein A3, 14  kDa, RF-A protein 3, RP-A p14, RPA14

Gene ID: 6119

UniProt: P35244

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA