FLASH antibody (C-Term)

Catalog No. ABIN214304

The Rabbit Polyclonal anti-FLASH antibody has been validated for WB, IHC, ICC and IHC (p). It is suitable to detect FLASH in samples from Human.

Quick Overview for FLASH antibody (C-Term) (ABIN214304)

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLASH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FLASH Antibody

Binding Specificity: C-Term

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (0.25 - 0.5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FLASH

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Alternative Name: CASP8AP2 / FLASH

Background: Name/Gene ID: CASP8AP2
Family: Apoptosis

Synonyms: CASP8AP2, CASP8 associated protein 2, CASP8-associated protein 2, CED-4, FLICE associated huge, FLICE-associated huge protein, KIAA1315, FLASH, FLASH homolog RIP25, Human FLASH, RIP25, Caspase 8 associated protein 2

Gene ID: 9994

UniProt: Q9UKL3

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity