FLASH antibody (N-Term)

Catalog No. ABIN2784233

This Rabbit Polyclonal antibody specifically detects FLASH in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Pig and Rabbit.It has been mentioned in 1 publication

Quick Overview for FLASH antibody (N-Term) (ABIN2784233)

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLASH antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-FLASH Antibody

Binding Specificity: N-Term

Purpose: CASP8AP2 Antibody - N-terminal region

Sequence: SLKKNISALI KTARVEINRK DEEISNLHQR LSEFPHFRNN HKTARTFDTV

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CASP8AP2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CASP8AP2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP50555-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-FLASH antibody (ABIN2784233)

Lee, Pi, Lee: "Buffer optimization for high resolution of human lung cancer tissue proteins by two-dimensional gel electrophoresis." in: Biotechnology letters, Vol. 31, Issue 1, pp. 31-7, (2008) (PubMed).

Target Details for FLASH

Target: FLASH (CASP8AP2) (CASP8 Associated Protein 2 (CASP8AP2))

Alternative Name: CASP8AP2

Background:

Background Information: This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.

Gene Name: caspase 8 associated protein 2

Alternative Symbols: CED-4, FLASH, RIP25

Protein Name: CASP8-associated protein 2

Molecular Weight: 223kDa

Gene ID: 9994

NCBI Accession: NP_036247

UniProt: Q9UKL3

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity