HDAC1 antibody (AA 466-482)

Catalog No. ABIN214334

The Rabbit Polyclonal anti-HDAC1 antibody is suitable to detect HDAC1 in samples from Human. It has been validated for WB, IHC, ELISA, IF and IHC (p).

Quick Overview for HDAC1 antibody (AA 466-482) (ABIN214334)

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HDAC1 Antibody

Binding Specificity: AA 466-482

Specificity: Amino acids 466-482 of Human HDAC-1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human HDAC1.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF (1:200 - 1:1000), IHC, IHC-P (20 μg/mL), WB (1:1000 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for HDAC1

Target: HDAC1 (Histone Deacetylase 1 (HDAC1))

Alternative Name: HDAC1

Background: Name/Gene ID: HDAC1
Family: Histone Deacetylase

Synonyms: HDAC1, Histone deacetylase 1, GON-10, HD1, RPD3, RPD3L1

Gene ID: 3065

UniProt: Q13547

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis