BNIP3L/NIX antibody (AA 77-92)

Catalog No. ABIN214415

The Rabbit Polyclonal anti-BNIP3L/NIX antibody is suitable to detect BNIP3L/NIX in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for BNIP3L/NIX antibody (AA 77-92) (ABIN214415)

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3L/NIX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BNIP3L/NIX Antibody

Binding Specificity: AA 77-92

Specificity: At least two isoforms of Bnip3L are known to exist.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BNIP3L/NIX

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Alternative Name: NIX / BNIP3L

Background: Name/Gene ID: BNIP3L
Family: Apoptosis

Synonyms: BNIP3L, NIP3-like protein X, NIP3L, NIX, BNIP3a, BNIP3H

Gene ID: 665

UniProt: O60238

Pathways: Autophagy