CD21 antibody (FITC)

Catalog No. ABIN2144534

The Mouse Monoclonal anti-CD21 antibody has been validated for FACS, IF, IP, IHC (fro) and ICC. It is suitable to detect CD21 in samples from Human.

Quick Overview for CD21 antibody (FITC) (ABIN2144534)

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD21 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: 4ABU32

Product Details anti-CD21 Antibody (FITC)

Characteristics: 4ABU32 reacts with CD21 protein, a 145 kDa receptor for C3d complement fragment and for Epstein-Barr virus (EBV), expressed on all mature B lymphocytes from sIg+ stage, then lost upon activation. It also present on follicular dendritic cells and some epithelia cells. It is reported CD21 weakly expressed on the subset of mature T -cells. CD21 plays a role in B cell activation and proliferation and is part of a large signal-transduction complex containing CD19, CD81and other molecules as regulator of complement activation.This clone is cross reactive with non-human primate .*CD21 is a phenotyping marker of lymphoma.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD21

Target: CD21 (CR2) (Complement Component (3d/Epstein Barr Virus) Receptor 2 (CR2))

Alternative Name: CD21

Pathways: Complement System