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CD47 antibody (FITC)

CD47 Reactivity: Human FACS Host: Mouse Monoclonal 4ACC2C6D4 FITC
Catalog No. ABIN2144616
  • Target See all CD47 Antibodies
    CD47
    Reactivity
    • 122
    • 45
    • 27
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 81
    • 62
    • 1
    • 1
    Mouse
    Clonality
    • 75
    • 68
    • 1
    Monoclonal
    Conjugate
    • 66
    • 12
    • 11
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This CD47 antibody is conjugated to FITC
    Application
    • 83
    • 82
    • 44
    • 41
    • 32
    • 26
    • 26
    • 18
    • 16
    • 14
    • 13
    • 11
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS)
    Characteristics
    4ACC2C6D4 reacts with CD47 ( gp42 ), a 45-55 kDa molecule, expressed on broad tissue and cells including hemopoietic cells , epithelial, endothelial cells and other tissue cells. CD47 antigen function on adhesion molecule and thrombospondin receptor.This clone is cross reactive with non-human primate.
    Clone
    4ACC2C6D4
    Isotype
    IgG1
    Top Product
    Discover our top product CD47 Primary Antibody
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Procedure
    • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
    • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
    • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
    • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
    • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
    • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
    • [Cell fixation: 2 % formaldehyde solution]
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    CD47
    Alternative Name
    CD47 (CD47 Products)
    Synonyms
    IAP antibody, MER6 antibody, OA3 antibody, 9130415E20Rik antibody, AA407862 antibody, AI848868 antibody, AW108519 antibody, B430305P08Rik antibody, Itgp antibody, CD47/IAP antibody, alkaline phosphatase, intestinal antibody, CD47 molecule antibody, CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody, Cd47 molecule antibody, ALPI antibody, CD47 antibody, Cd47 antibody
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