SIRPA antibody (C-Term)

Catalog No. ABIN214476

The Rabbit Polyclonal anti-SIRPA antibody (ABIN214476) specifically detects SIRPA in WB, IHC, ICC and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SIRPA antibody (C-Term) (ABIN214476)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIRPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRPA Antibody

Binding Specificity: C-Term

Specificity: Recognizes SIRP alpha 1, 2 and 3.

Purification: Purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (2.5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172A / SIRPA

Background: Name/Gene ID: SIRPA

Synonyms: SIRPA, CD172A, CD172a antigen, Inhibitory receptors shps-1, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, p84, SHPS-1, SHPS1, SIRP, Sirp-alpha-1, Sirp-alpha-3, Sirp-alpha1, MYD1, SIRPalpha, SIRPalpha2, BIT, MFR, MYD-1, PTPNS1, SHP substrate 1, Sirp-alpha-2

Gene ID: 140885

UniProt: P78324