FEM1B antibody (AA 609-622)

Catalog No. ABIN214493

The Rabbit Polyclonal anti-FEM1B antibody has been validated for WB, IHC and IHC (p). It is suitable to detect FEM1B in samples from Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Bat, Monkey, Pig and Hamster.

Quick Overview for FEM1B antibody (AA 609-622) (ABIN214493)

Target: FEM1B (Fem-1 Homolog B (FEM1B))

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Bat, Monkey, Pig, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEM1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FEM1B Antibody

Binding Specificity: AA 609-622

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig (100%) Opossum (86%).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.5 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FEM1B

Target: FEM1B (Fem-1 Homolog B (FEM1B))

Alternative Name: FEM1B

Background: Name/Gene ID: FEM1B

Synonyms: FEM1B, F1AA, FEM-1 (C.elegans) homolog b, FEM1-beta, FIAA, KIAA0396, F1A-ALPHA, Fem-1 homolog b (C. elegans), Protein fem-1 homolog B

Gene ID: 10116

UniProt: Q9UK73