GJB2 antibody (Cytoplasmic Domain)

Catalog No. ABIN215079

This anti-GJB2 antibody is a Rabbit Polyclonal antibody detecting GJB2 in WB, ELISA, IHC, IF and IHC (fro). Suitable for Rat.

Quick Overview for GJB2 antibody (Cytoplasmic Domain) (ABIN215079)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GJB2 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Reacts predominantly with Connexin 26. By immunoblot analysis, a 26kD protein from mouse liver membrane, mouse brain extract and rat brain extract was detected (note: samples were not boiled prior to running SDS-PAGE). A few cross-reactive bands were detected in the 45-50kD range. The identity of these proteins has yet to be confirmed, however, it is possible these bands represent dimeric forms of the Connexin 26 protein. Immunohistochemical staining of frozen sections of human cochlea showed positive reactivity for Connexin 26.

Purification: Immunoaffinity purified

Immunogen: Peptide corresponding to a portion of the cytoplasmic loop of rat connexin 26.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, WB

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. ELISA: 0.1-1.0 mg/mL. Western Blot: 0.5 mg/mL. Immunohistochemistry (frozen): 10 mg/mL. Immunofluorescence.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: GJB2 / CX26 / Connexin 26

Background: Name/Gene ID: GJB2
Subfamily: Connexin
Family: Ion Channel

Synonyms: GJB2, Connexin 26, Connexin-26, DFNB1A, DFNA3, DFNB1, Gap junction protein beta 2, KID, HID, PPK, NSRD1, CX26, DFNA3A

Gene ID: 2706

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization