GAP43 antibody

Catalog No. ABIN215199

The Mouse Monoclonal anti-GAP43 antibody (ABIN215199) specifically detects GAP43 in WB, IHC and IP. The antibody is reactive with Human, Rat, Cat and Hamster samples.

Quick Overview for GAP43 antibody (ABIN215199)

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Reactivity: Human, Rat, Cat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-GAP43 Antibody

Specificity: Recognizes GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein's phosphorylation state. Species Reactivity: Human, rat, feline and hamster.

Purification: Purified

Immunogen: GAP-43 purified from rat brain.

Isotype: IgG1

Application Details

Application Notes: Approved: IHC (0.1 - 0.5 μg/mL), IP, WB (0.1 - 0.5 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Immunohistochemistry: 0.1-0.5 μg/mL on 4 % paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1 % Triton X- 100 to incubation buffer will increase cellular permeability. Western blot: 0.1-0.5 μg/mL using rat cerebral preparation (7.5 % non-gradient denaturing gel). Will detect a single 48kD band. Western Blot: 1:1000 on 10 μg of rat brain lysates.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43

Background: Name/Gene ID: GAP43
Family: Neuromodulin

Synonyms: GAP43, Axonal membrane protein GAP-43, B-50, Growth associated protein 43, Growth-associated protein 43, Neural phosphoprotein B-50, Neuromodulin, PP46, GAP-43, Protein F1

Gene ID: 2596

UniProt: P17677