HSPD1 antibody (AA 383-447)

Catalog No. ABIN215204

The Mouse Monoclonal anti-HSPD1 antibody has been validated for WB, FACS, ELISA and IP. It is suitable to detect HSPD1 in samples from Human, Mouse, Rat, Chicken, Monkey, Hamster, Dog, Cow, Rabbit, Pig, Sheep, Guinea Pig, Drosophila melanogaster and Xenopus laevis.

Quick Overview for HSPD1 antibody (AA 383-447) (ABIN215204)

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivity: Human, Mouse, Rat, Chicken, Monkey, Hamster, Dog, Cow, Rabbit, Pig, Sheep, Guinea Pig, Drosophila melanogaster, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPD1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Product Details anti-HSPD1 Antibody

Binding Specificity: AA 383-447

Specificity: Recognizes human HSP60. Species cross-reactivity: Mouse, rat, bovine, canine, chicken, Drosophila, guinea pig, hamster, monkey, porcine, rabbit, sheep and Xenopus. Shows unique specificity for mammalian and insect Hsp60 and does not cross react with bacterial or yeast Hsp60 counterparts.

Purification: Protein G purified

Immunogen: Human Hsp60 produced through recombinant DNA methods in E. coli. Deletion mutant analysis indicates that the epitope recognized by LK-1 is located between residues 383-447 of the human Hsp60 sequence.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:100), IP (1:80), WB (1:1000)

Usage: Suitable for use in Western Blot, Immunoprecipitation, ELISA, and Flow Cytometry. Western Blot: 1:1000. Immunoprecipitation: 1:80. Flow Cytometry: 1:100. Positive control: HeLa Heat Shocked Cell Lysate, Cat# H1838-01.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.9 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSPD1

Target: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Alternative Name: HSPD1 / HSP60

Background: Name/Gene ID: HSPD1

Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL

Gene ID: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity