ATP1A1 antibody (AA 338-518)

Catalog No. ABIN215645

The Rabbit Polyclonal anti-ATP1A1 antibody is suitable to detect ATP1A1 in samples from Rat and Cow. It has been validated for WB, IHC, IP, ICC and IHC (fro).

Quick Overview for ATP1A1 antibody (AA 338-518) (ABIN215645)

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ATP1A1 Antibody

Binding Specificity: AA 338-518

Specificity: Specific for the a-1 subunit isoform at Mr of 110kD. Species cross-reactivity: Rat and bovine. Does not cross-react with rabbit.

Purification: Protein G purified

Immunogen: Fusion protein containing residues 338-518 of the a-1 subunit, as deduced from the cDNA.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (5 μg/mL), IHC, IHC-Fr, IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Previous lots were successfully used to immunoprecipitate the a-1 subunit from of rat brain microsomes. Immunocytochemistry: 5 μg/mL detects the Na+ /K+ ATPase a-1 subunit in frozen sections of rat kidney and brain, which were fixed in 4 % paraformaldehyde.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1

Background: Name/Gene ID: ATP1A1
Subfamily: ATPase - P type, type IIC
Family: Transporter

Synonyms: ATP1A1, Na+, K+ ATPase alpha subunit, Na,K-ATPase alpha-1 subunit, Na+/K+ ATPase 1, Sodium pump 1, Sodium pump subunit alpha-1

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process