C3 antibody

Catalog No. ABIN215710

This Mouse Monoclonal antibody specifically detects C3 in WB, IHC and IHC (fro). It exhibits reactivity toward Human, Guinea Pig and Rabbit.

Quick Overview for C3 antibody (ABIN215710)

Target: C3 (Complement Component 3 (C3))

Reactivity: Human, Guinea Pig, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: H13

Product Details anti-C3 Antibody

Specificity: Recognizes a C3a-determinant (C3 alpha-chain) which is present also on native C3. Allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment, C3b or C are negative with this clone. The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited, similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. Species cross-reactivity: Guinea pig and rabbit C3/C3a.

Purification: Protein A purified

Immunogen: Human complement component C3.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IHC, IHC-Fr, WB

Usage: Suitable for use in Immunohistochemistry (frozen sections) and Western blotting. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays research purposes.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mL sterile ddH20

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 0.5 % BSA, 0.9 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Lyophilized and resuspended forms: store at -20°C.

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: Complement C3

Background: Name/Gene ID: C3

Synonyms: C3, AHUS5, ARMD9, Complement component C3, CPAMD1, Complement C3, ASP, Complement component 3

Gene ID: 718

UniProt: P01024

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling