Dopamine Receptor d1 antibody (AA 403-415)

Catalog No. ABIN215780

The Rabbit Polyclonal anti-Dopamine Receptor d1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Dopamine Receptor d1 in samples from Rat.

Quick Overview for Dopamine Receptor d1 antibody (AA 403-415) (ABIN215780)

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dopamine Receptor d1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Dopamine Receptor d1 Antibody

Binding Specificity: AA 403-415

Specificity: Recognizes 2 rat proteins of 50 and 75kD in solubilized hippocampal membranes by Western blot. The 75kD band was not visible in striatum. Approximately 100-400 μg of protein/lane should be loaded to visualize the 75kD band. In IHC, D1R has been localized in various regions of the rat brain. Species sequence Homology: porcine or bovine (100 % ), human (78 %) and mouse (92 %).

Purification: Affinity purified

Immunogen: A 13 amino acid peptide sequence (amino acids 403-415) from rat D1a (1). This peptide is predicted to be cytoplasmic.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), IHC (2 - 10 μg/mL), WB (1 - 10 μg/mL)

Usage: Suitable for use in Western blot, Immunohistochemistry, and ELISA. Western Blot: 1-10 μg/mL (Chemiluminescence technique) Immunohistochemistry: 2-10 μg/mL on paraformaldehyde fixed sections. ELISA: 0.5-1.0 μg/mL (1 μg/mL D1A peptide/well).

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris,  pH 7.5, 0.2 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Dopamine Receptor d1

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Alternative Name: DRD1 / Dopamine Receptor D1

Background: Name/Gene ID: DRD1
Subfamily: Dopamine
Family: GPCR

Synonyms: DRD1, D(1A) dopamine receptor, D1a dopamine receptor, Dopamine d-1 receptor, Dopamine receptor d1a, DRD1A, DADR, Dopamine d1a receptor, Dopamine receptor D1, Dopamine 1 receptor, Dopamine type 1 receptor, D-1 dopamine receptor, D1 dopamine receptor, D1a receptor, Dopamine D1 receptor

Gene ID: 1812

UniProt: P21728

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity