Metabotropic Glutamate Receptor 1 antibody (AA 1180-1199)

Catalog No. ABIN216022

This Rabbit Polyclonal antibody specifically detects Metabotropic Glutamate Receptor 1 in WB, IHC, ICC and IHC (fro). It exhibits reactivity toward Mouse, Rat, Cow, Bat, Dog, Hamster and Horse.

Quick Overview for Metabotropic Glutamate Receptor 1 antibody (AA 1180-1199) (ABIN216022)

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivity: Mouse, Rat, Cow, Bat, Dog, Hamster, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Metabotropic Glutamate Receptor 1 Antibody

Binding Specificity: AA 1180-1199

Specificity: Metabotropic glutamate receptor 1 alpha (mGluR1a). Western blot analysis shows the antibody labels a single band migrating at Mr = 140000.

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Opossum (100%) Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit, Zebrafish (95%) Chicken, Stickleback (90%) Platypus, Xenopus (85%).

Purification: Affinity purified

Immunogen: Carboxy terminus peptide of rat mGluR1a conjugated to KLH with glutaraldehyde (PNVTYASVILRDYKQSSSTL). Percent identity by BLAST analysis: Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Opossum (100%), Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit, Zebrafish (95%), Chicken, Stickleback (90%), Platypus, Xenopus (85%).

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, WB

Usage: Western blot analysis can be done at final concentrations of 0.25-1.0 μg/mL. Immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations recommended are 0.5-2.0 μg/mL. The antibody has not been tested for use in immunoprecipitation. However, antibodies to the carboxy termini of other glutamate receptors are very effective in immunoprecipitation.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 500 μL sterile ddH2O

Concentration: Lot specific

Buffer: Lyophilized from 0.02M PBS,  pH 7.6, 0.1 % BSA, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Alternative Name: GRM1 / MGLUR1

Background: Name/Gene ID: GRM1
Subfamily: Metabotropic glutamate
Family: GPCR

Synonyms: GRM1, GRM1A, GPRC1A, MGlu1, MGLUR1, SCAR13, MGLUR1A

Gene ID: 2911

UniProt: Q13255