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GPR84 antibody (AA 21-120)

Name: GPR84 antibody (AA 21-120)
SKU: ABIN2173922
Price: 384.62 USD
Availability: InStock

GPR84 Reactivity: Human, Mouse, Rabbit WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Mouse heart lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Mouse bone and brain lysates probed with GPCR EX33 Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded human glioma tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

Paraformaldehyde-fixed, paraffin embedded human colon carcinoma, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with GPCR EX33 Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IHC (p)

Mouse thymus lysates probed with GPCR EX33 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).

FACS

5 images

Catalog No. ABIN2173922

Datasheet as PDF

Target See all GPR84 Antibodies

GPR84 (G Protein-Coupled Receptor 84 (GPR84))
Binding Specificity
16

7

5

5

5

5

3

2

2

2

1

1

1

1

1

1

1

1

1
AA 21-120
Reactivity
44

25

8

4

4

2

1

1

1

1
Human, Mouse, Rabbit
Host
58

3
Rabbit
Compatible Secondaries
Clonality
59

2
Polyclonal
Conjugate
20

6

4

4

4

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1
This GPR84 antibody is un-conjugated

Application

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rabbit

Predicted Reactivity

Cow,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human GPCR EX33 protein

Isotype

IgG
anti-G Protein-Coupled Receptor 84 (GPR84) (Cytoplasmic Domain) antibody
GPR84 Reactivity: Human ELISA, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 208-316) antibody
GPR84 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 5C3 unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (2nd Extracellular Loop), (AA 166-180) antibody
GPR84 Reactivity: Human, Mouse, Rat WB, FACS, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (N-Term) antibody
GPR84 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Extracellular Domain) antibody
GPR84 Reactivity: Human, Horse, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (Transmembrane Domain) antibody
GPR84 Reactivity: Human IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) antibody
GPR84 Reactivity: Mouse, Rat WB Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 202-320) antibody
GPR84 Reactivity: Human ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 1-100) antibody
GPR84 Reactivity: Mouse WB, ELISA, FACS Host: Rabbit Polyclonal unconjugated

anti-G Protein-Coupled Receptor 84 (GPR84) (AA 265-293) antibody
GPR84 Reactivity: Human WB Host: Rabbit Polyclonal RB36053 unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Alternative Name

GPCR EX33 (GPR84 Products)

Synonyms

EX33 antibody, GPCR4 antibody, GPR84 antibody, sb:eu355 antibody, zgc:171802 antibody, G protein-coupled receptor 84 antibody, G protein-coupled receptor 84 S homeolog antibody, GPR84 antibody, Gpr84 antibody, gpr84 antibody, gpr84.S antibody

Background

Synonyms: EX33, GPCR4, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84
Background: Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system.

Gene ID

53831

UniProt

Q9NQS5