HCST antibody (AA 19-48) (AbBy Fluor® 488)

Catalog No. ABIN2175293

The Rabbit Polyclonal anti-HCST antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect HCST in samples from Human and Rat.

Quick Overview for HCST antibody (AA 19-48) (AbBy Fluor® 488) (ABIN2175293)

Target: HCST (Hematopoietic Cell Signal Transducer (HCST))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HCST antibody is conjugated to AbBy Fluor® 488

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-HCST Antibody (AbBy Fluor® 488)

Binding Specificity: AA 19-48

Cross-Reactivity: Human, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HCST

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HCST

Target: HCST (Hematopoietic Cell Signal Transducer (HCST))

Alternative Name: Hcst

Background:

Synonyms: DAP10, KAP10, PIK3AP, Hematopoietic cell signal transducer, DNAX-activation protein 10, Membrane protein DAP10, Transmembrane adapter protein KAP10, HCST, UNQ587/PRO1157

Background: Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells, this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs), these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells, indeed, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells.

Gene ID: 10870

UniProt: Q9UBK5