Nestin antibody (AA 681-750) (PE)

Catalog No. ABIN2177881

This Rabbit Polyclonal antibody specifically detects Nestin in FACS, IF (p) and IF (cc). It exhibits reactivity toward Rat.It has been mentioned in 1 publication

Quick Overview for Nestin antibody (AA 681-750) (PE) (ABIN2177881)

Target: Nestin (NES)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nestin antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-Nestin Antibody (PE)

Binding Specificity: AA 681-750

Purpose: Nestin Polyclonal Antibody, PE Conjugated

Cross-Reactivity: Mouse

Predicted Reactivity: Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from rat Nestin

Isotype: IgG

Application Details

Application Notes: FCM(1:20-100), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-Nestin antibody (PE) (ABIN2177881)

Yamamizu, Fujihara, Tachibana, Katayama, Takahashi, Hara, Imai, Shinkai, Yamashita: "Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a." in: Cell stem cell, Vol. 10, Issue 6, pp. 759-70, (2012) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background:

Synonyms: ESTM 46, FLJ 21841, FLJ21841, Intermediate filament protein, Nbla00170, Nes, NEST_RAT, Nestin.

Background: Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.

Gene ID: 25491

UniProt: P21263