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IL-3 antibody

The Rat Monoclonal anti-IL-3 antibody has been validated for WB and ELISA. It is suitable to detect IL-3 in samples from Mouse.
Catalog No. ABIN218087

Quick Overview for IL-3 antibody (ABIN218087)

Target

See all IL-3 Antibodies
IL-3 (Interleukin 3 (IL-3))

Reactivity

  • 87
  • 43
  • 28
  • 6
  • 1
  • 1
Mouse

Host

  • 98
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Rat

Clonality

  • 104
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Monoclonal

Conjugate

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  • 2
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  • 2
  • 1
  • 1
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  • 1
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This IL-3 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA
  • Specificity

    Recognizes mouse Interleukin-3 (IL-3).

    Purification

    Purified

    Immunogen

    COS-expressed recombinant mouse IL-3.

    Isotype

    IgG2a kappa
  • Application Notes

    Approved: ELISA, WB

    Usage: ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-3 protein levels.2,3,5 Biotinylated antibody should be paired with purified antibody (Cat. Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-3 under reducing conditions.

    Comment

    Target Species of Antibody: Mouse

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    100 mM borate buffered saline,  pH 8.0. No preservatives or amine-containing buffer salts added.

    Preservative

    Without preservative

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • Target

    IL-3 (Interleukin 3 (IL-3))

    Alternative Name

    IL3

    Background

    Name/Gene ID: IL3
    Family: Hormone

    Synonyms: IL3, Hematopoietic growth factor, IL-3, Mast-cell growth factor, MULTI-CSF, P-cell-stimulating factor, Interleukin-3, Mast cell growth factor, MCGF, P-cell stimulating factor

    Gene ID

    3562

    UniProt

    P08700

    Pathways

    JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
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