IL-4 antibody

Catalog No. ABIN218112

This anti-IL-4 antibody is a Rat Monoclonal antibody detecting IL-4 in WB, ELISA, FACS and IF. Suitable for Mouse.

Quick Overview for IL-4 antibody (ABIN218112)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-IL-4 Antibody

Specificity: Recognizes mouse Interleukin-4 (IL-4).

Purification: Purified

Immunogen: E. coli-expressed recombinant mouse IL-4.

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, Flo, IF, WB

Usage: ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse IL-4 protein levels.1-4 Purified antibody should be paired with biotinylated antibody (Cat. Immunofluorescence/. Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-4-positive cells within mixed cell populations. Recombinant mouse IL-4, unlabeled Rat IgG2b should be used as a specificity control. Western Blot: Useful for Western blot applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant mouse IL-4 under reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM borate buffered saline,  pH 8.2. No preservatives or amine-containing buffer salts added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL4

Background: Name/Gene ID: IL4
Family: Hormone

Synonyms: IL4, B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, BCGF1, BSF-1, Binetrakin, BCGF-1, Interleukin 4, Interleukin-4, Pitrakinra, BSF1, IL-4

Gene ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation