IL-6 antibody (Biotin)

Catalog No. ABIN218130

This anti-IL-6 antibody is a Rat Monoclonal antibody detecting IL-6 in WB, ELISA and IP. Suitable for Human.

Quick Overview for IL-6 antibody (Biotin) (ABIN218130)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-IL-6 Antibody (Biotin)

Specificity: Recognizes human Interleukin-6 (IL-6).

Purification: Purified

Immunogen: COS-7-expressed recombinant human IL-6.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IP, WB

Usage: ELISA Detection: Monoclonal antibody is useful as a detection antibody in a sandwich ELISA for quantifying human IL-6 protein levels.1-3 Biotinylated antibody should be paired with purified antibody as the capture antibody, with purified recombinant human IL-6 as the standard. Western Blot: The purified antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-6 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background: Name/Gene ID: IL6
Family: Hormone

Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

Gene ID: 3569

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome