IL-6 antibody

Catalog No. ABIN218146

This Rat Monoclonal antibody specifically detects IL-6 in WB, IHC, ELISA, FACS and Neut. It exhibits reactivity toward Mouse.

Quick Overview for IL-6 antibody (ABIN218146)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Product Details anti-IL-6 Antibody

Specificity: Recognizes natural and recombinant mouse IL-6.

Purification: Protein A/G purified

Immunogen: Recombinant mouse IL-6.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1 - 5 μg/mL), Flo, IHC, Neut, WB

Usage: Suitable for use in ELISA, Neutralization, Flow Cytometry, Immunohistochemistry and Western Blot. ELISA:1-5 μg/mL (dilute immediately before use). Use 100 μL to coat each well. Flow Cytometry: 0.1-0.5 μg/1E6 splenocytes stimulated with PMA and ionophore for 6 hours. Optimum dilutions to be determined by the researcher. Hybridoma: Rat spleen cells fused with P3X63-AG8.653 myeloma. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, no preservatives.

Preservative: Without preservative

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background: Name/Gene ID: IL6
Family: Hormone

Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

Gene ID: 3569

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome