IL-13 antibody (Biotin)

Catalog No. ABIN218189

The Rat Monoclonal anti-IL-13 antibody has been validated for WB and ELISA. It is suitable to detect IL-13 in samples from Human.

Quick Overview for IL-13 antibody (Biotin) (ABIN218189)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-13 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL-13 Antibody (Biotin)

Specificity: Recognizes human Interleukin-13 (IL-13).

Purification: Purified

Immunogen: COS-expressed recombinant human IL-13.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, WB

Usage: ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying human IL-13 protein levels.1,2 Biotinylated antibody should be paired with purified antibody (Cat. Western Blot: Useful for Western blot applications. A concentration of 1-5 μg/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of 100 ng/lane of recombinant human IL-13 under reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Background: Name/Gene ID: IL13
Family: Hormone

Synonyms: IL13, ALRH, BHR1, Interleukin-13, NC30, p600, IL-13, Interleukin 13

Gene ID: 3596

UniProt: P35225

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport