ZAP70 antibody (pTyr493)

Catalog No. ABIN218908

This Rabbit Polyclonal antibody specifically detects ZAP70 in WB, FACS, ELISA and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for ZAP70 antibody (pTyr493) (ABIN218908)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunoprecipitation (IP)

Product Details anti-ZAP70 Antibody

Binding Specificity: pTyr493

Specificity: Detects endogenous levels of Tyr493 phosphorylated human Zap-70 (Mr 70kD). Species cross-reactivity: Predicted to cross-react with mouse based on sequence homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Tyr493 of human Zap-70 (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:100), IP (1:100), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Flow Cytometry. Western Blot: 1:1000. Immunoprecipitation: 1:100. Flow Cytometry: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background: Name/Gene ID: ZAP70
Subfamily: Syk/ZAP70
Family: Protein Kinase

Synonyms: ZAP70, 70 kDa zeta-associated protein, SRK, STCD, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, TZK, ZAP-70, Zeta-associated protein 70

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway