APEX1 antibody

Catalog No. ABIN219055

This Rabbit Polyclonal antibody specifically detects APEX1 in WB, IHC, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for APEX1 antibody (ABIN219055)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APEX1 Antibody

Specificity: Recognizes human APE. Detects a band of ~37kD by Western Blot. Species cross-reactivity: mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Recombinant human APE.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:200), IHC, IHC-Fr, IHC-P, WB (1:300 - 1:1000)

Usage: Suitable for use in Immunocytochemistry, Western Blot and Immunohistochemistry. Also suitable for use in Apoptosis assay (following TUNEL assay using Boehringer-Mannheim TUNEL assay kit). Western Blot: 1:300-1:1000 on HL60 and HeLa cells using ECL. Antibody may be used 1-4X in the binding solution depending on the cells/cell lines. Immunohistochemistry: 1:100-1:200 on frozen and paraffin sections from human and rat tissues. Immunocytochemistry: 1:100-1:200 on cytospin preparations of cells fixed in paraformaldehyde. Apoptosis (TUNEL) assay: perform antibody staining AFTER the. TUNEL assay.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS. Contains no preservatives.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 / APE1

Background: Name/Gene ID: APEX1

Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1

Gene ID: 328

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus