MBL-C antibody
Reactivity: Mouse
WB, IA, IF, FACS
Host: Rat
Monoclonal
14D12
unconjugated
1 image
(4 references)-
- Target See all MBL-C Antibodies
- MBL-C (Mannose-Binding Lectin C)
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This MBL-C antibody is un-conjugated
- Application
- Western Blotting (WB), Immunoassay (IA), Immunofluorescence (IF), Flow Cytometry (FACS)
- Sterility
- 0.2 μm filtered
- Clone
- 14D12
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- Application Notes
- For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. It is recommended that solutions with a calcium concentration of 1 mM are used (14D12 is a calcium- dependent antibody). Positive Mouse serum, Kidney tissue control
- Restrictions
- For Research Use only
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- Buffer
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "Binding to complement factors and activation of the alternative pathway by Acanthamoeba." in: Immunobiology, Vol. 216, Issue 1-2, pp. 225-33, (2010) (PubMed).
: "Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans." in: Molecular immunology, Vol. 45, Issue 15, pp. 3934-41, (2008) (PubMed).
: "Binding of mouse mannan-binding lectins to different bacterial pathogens of mice." in: Veterinary immunology and immunopathology, Vol. 118, Issue 1-2, pp. 129-33, (2007) (PubMed).
: "Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses." in: Scandinavian journal of immunology, Vol. 53, Issue 5, pp. 489-97, (2001) (PubMed).
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: "Binding to complement factors and activation of the alternative pathway by Acanthamoeba." in: Immunobiology, Vol. 216, Issue 1-2, pp. 225-33, (2010) (PubMed).
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- Target
- MBL-C (Mannose-Binding Lectin C)
- Alternative Name
- Mannose Binding Lectin C (MBL-C Products)
- Synonyms
- L-MBP antibody, MBL antibody, MBL-C antibody, MBP-C antibody, COLEC2 antibody, mannose-binding lectin (protein C) 2 antibody, mannose-binding lectin family member 3, pseudogene antibody, Mbl2 antibody, MBL3P antibody
- Background
- Mannose binding lectin (MBL), also called mannose- or mannan-binding protein (MBP), is a member of the group of collectins. MBL is an important pattern-recognition receptor in the innate immune system. The protein mediates innate immune responses, such as activation of the complement lectin pathway and phagocytosis, to help fight infections. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. Binding of MBL leads to the activation of MBL- associated serine proteases (MASP's). Activated MASP-2 cleaves C4 and C2 in a similar way as C1s do for the classical pathway (CP) leading to the formation of C4b2a, cleavage of the classical pathway convertase C3, and eventually complement activation up to the formation of the membrane attack complex. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. MBL is predominantly synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The mouse forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold compared to that of MBL-A. MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. The monoclonal antibody 14D12 is a calcium-dependent antibody. Aliases MBL, L-MB, MBL-C, MBP-C, Mbl2 Immunogen Purified MBL-C
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