PVRL2 antibody

Catalog No. ABIN2191800

This anti-PVRL2 antibody is a Rat Monoclonal antibody detecting PVRL2 in WB, FACS, IP, IHC (fro) and IA. Suitable for Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PVRL2 antibody (ABIN2191800)

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This PVRL2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Clone: 502-57

Product Details anti-PVRL2 Antibody

Application Details

Application Notes: For flow cytometry, Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. Fixation with PFA or acetone/methanol is recommended.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-PVRL2 antibody (ABIN2191800)

Kawabe, Nakanishi, Asada, Fukuhara, Morimoto, Takeuchi, Takai: "Pilt, a novel peripheral membrane protein at tight junctions in epithelial cells." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48350-5, (2001) (PubMed).

Sakisaka, Nakanishi, Takahashi, Mandai, Miyahara, Satoh, Takaishi, Takai: "Different behavior of l-afadin and neurabin-II during the formation and destruction of cell-cell adherens junction." in: Oncogene, Vol. 18, Issue 8, pp. 1609-17, (1999) (PubMed).

Target Details for PVRL2

Target: PVRL2 (Poliovirus Receptor-Related 2 (Herpesvirus Entry Mediator B) (PVRL2))

Alternative Name: Nectin-2

Background: Nectin (originally isolated as poliovirus receptor-related protein (PRR)) is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectin is colocalized with afadin at cadherin-based cell-cell adherence junctions in various tissues and cell lines. The nectin family consist of nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 is a plasma membrane adhesion molecule localized at adherens junctions which is widely expressed in various cell lines including neuronal, endothelial, epithelial and hematopoietic cells. Next to its role in adherens junctions it functions as alphaherpus virus receptor and acts also as intercellular adhesion molecule and pseudorabies virus receptor. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal antibody 502-57 has been raised against the extracellular domain of mouse nectin-2. The antibody cross reacts with human nectin-2.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization