FABP3 antibody

Catalog No. ABIN2191880

The Mouse Monoclonal anti-FABP3 antibody has been validated for WB, IP and IA. It is suitable to detect FABP3 in samples from Human. There are 5+ publications available.

Quick Overview for FABP3 antibody (ABIN2191880)

Target: FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FABP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunoassay (IA)

Clone: 66E2

Product Details anti-FABP3 Antibody

Cross-Reactivity (Details): Cross reactivity: Rat H-FABP : Yes, Mouse H-FABP : Yes, Swine H-FABP : Yes, Human B-FABP Average (in ELISA) Human A-FABP : No, Human I-FABP : No, Human L-FABP : No

Sterility: 0.2 μm filtered

Isotype: IgG1

Application Details

Application Notes: For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.. Positive Heart cells, or recombinant H-FABP control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-FABP3 antibody (ABIN2191880)

Zhen, Berna, Jin, Pritt, Watson, Ackermann, Hale: "Quantification of heart fatty acid binding protein as a biomarker for drug-induced cardiac and musculoskeletal necroses." in: Proteomics. Clinical applications, Vol. 1, Issue 7, pp. 661-71, (2010) (PubMed).

Zimmermann-Ivol, Burkhard, Le Floch-Rohr, Allard, Hochstrasser, Sanchez: "Fatty acid binding protein as a serum marker for the early diagnosis of stroke: a pilot study." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 1, pp. 66-72, (2004) (PubMed).

Pelsers, Hanhoff, Van der Voort, Arts, Peters, Ponds, Honig, Rudzinski, Spener, de Kruijk, Twijnstra, Hermens, Menheere, Glatz: "Brain- and heart-type fatty acid-binding proteins in the brain: tissue distribution and clinical utility." in: Clinical chemistry, Vol. 50, Issue 9, pp. 1568-75, (2004) (PubMed).

Guillaume, Zimmermann, Burkhard, Hochstrasser, Sanchez: "A potential cerebrospinal fluid and plasmatic marker for the diagnosis of Creutzfeldt-Jakob disease." in: Proteomics, Vol. 3, Issue 8, pp. 1495-9, (2003) (PubMed).

Roos, Eymann, Symannek, Duppenthaler, Wodzig, Pelsers, Glatz: "Monoclonal antibodies to human heart fatty acid-binding protein." in: Journal of immunological methods, Vol. 183, Issue 1, pp. 149-53, (1995) (PubMed).

Target Details for FABP3

Target: FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart (FABP3))

Alternative Name: Fatty Acid-Binding Protein, Heart

Background: The monoclonal antibody 66E2 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long chain fatty acids. They are abundantly present in various cell types and play an important role in the intracellular utilization of fatty acids, transport and metabolism. There are at least nine distinct types of FABP, each showing a specific pattern of tissue expression. Due to its small size, FABP leaks rapidly out of ischemically damaged necrotic cells leading to a rise in serum levels. Ischemically damaged tissues are characterized histologically by absence (or low presence) of FABP facilitating recognition of such areas. H-FABP is localized in the heart, skeletal and smooth muscle, mammary epithelial cells, aorta, distal tubules of the kidney, lung, brain, placenta, and ovary. It is also useful as marker for brain damage. Furthermore, this antibody is useful for the purification of H-FABP. Aliases Fatty acid-binding protein 3, FABP3, Muscle fatty acid-binding protein, Mammary-derived growth inhibitor Immunogen Purified human H-FABP

Pathways: Monocarboxylic Acid Catabolic Process