Complement C5a/C5a Des-Arg (Neo-epitope des-Arg) antibody

Catalog No. ABIN2191949

Product Details

Target: Complement C5a/C5a Des-Arg

Binding Specificity: Neo-epitope des-Arg

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Functional Studies (Func), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Sterility: 0.2 μm filtered

Clone: 2942

Application Details

Application Notes: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Eye tissue of patient with AMD (Ref.1) control Negative Eye tissue of patient without AMD (Ref.1) control

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References

Nozaki, Raisler, Sakurai, Sarma, Barnum, Lambris, Chen, Zhang, Ambati, Baffi, Ambati: "Drusen complement components C3a and C5a promote choroidal neovascularization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 7, pp. 2328-33, (2006) (PubMed).

Target Details

Target: Complement C5a/C5a Des-Arg

Background: Monoclonal antibody 2942 reacts with a neo-epitope on human C5a/C5a des-Arg that is formed by proteolytic cleavage of C5 into C5a and C5b during complement activation. C5a is a single chain glycopeptide composed of 74-amino-acids, which is rapidly converted to the less potent derivate C5a des-Arg upon removal of the C-terminal arginine by carboxypeptidase B. C5a acts as a potent anaphylatoxin by binding to its high affinity receptor C5aR (or CD88) on the membrane of target cells inducing smooth muscle contraction, increased vascular permeability, degranulation of mast cells and basophils, and the release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. C5a is involved in inflammatory reactions seen in gram-negative bacterial sepsis, trauma, ischemic heart disease, post-dialysis syndrome and a variety of autoimmune diseases. Elevation of C5a is associated with increased cardiovascular risk in patients with advanced atherosclerosis. Also, C5a is closely associated with the capillary leak syndrome in leukemic children after bone marrow transplantation. C5a is also a marker in urine for predicting the onset of acute graft rejection after kidney transplantation and has been shown to be present in extracellular deposits in patients suffering from age-related macular degeneration (AMD).