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FHL2 antibody

FHL2 Reactivity: Human WB, IF, IHC (p), IHC (fro) Host: Mouse Monoclonal F4B2-B11 unconjugated
Catalog No. ABIN2192004
  • Target See all FHL2 Antibodies
    FHL2 (Four and A Half LIM Domains 2 (FHL2))
    Reactivity
    • 43
    • 31
    • 17
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 35
    • 7
    • 2
    Mouse
    Clonality
    • 29
    • 14
    Monoclonal
    Conjugate
    • 26
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This FHL2 antibody is un-conjugated
    Application
    • 25
    • 11
    • 9
    • 8
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Sterility
    0.2 μm filtered
    Clone
    F4B2-B11
    Top Product
    Discover our top product FHL2 Primary Antibody
  • Application Notes
    For immunohistology and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.
    Restrictions
    For Research Use only
  • Buffer
    PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
    Expiry Date
    12 months
  • Martin, Schneider, Janetzky, Waibler, Pandur, Kühl, Behrens, von der Mark, Starzinski-Powitz, Wixler: "The LIM-only protein FHL2 interacts with beta-catenin and promotes differentiation of mouse myoblasts." in: The Journal of cell biology, Vol. 159, Issue 1, pp. 113-22, (2002) (PubMed).

  • Target
    FHL2 (Four and A Half LIM Domains 2 (FHL2))
    Alternative Name
    Fhl2 (FHL2 Products)
    Synonyms
    AAG11 antibody, DRAL antibody, FHL-2 antibody, SLIM-3 antibody, SLIM3 antibody, C76204 antibody, FHL2 antibody, zgc:92428 antibody, zgc:103466 antibody, four and a half LIM domains 2 antibody, four and a half LIM domains 2a antibody, four and a half LIM domains 2b antibody, FHL2 antibody, Fhl2 antibody, fhl2 antibody, fhl2a antibody, fhl2b antibody
    Background
    The monoclonal antibody F4B2-B11 reacts specifically with the LIM-only protein FHL2. Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine- rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl- 1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows specific interaction with beta-catenin, which requires the intact structure of all four LIM domains of FHL2 and the N-terminus plus the first armadillo repeat region of beta-catenin. FHL2 is a muscle-specific repressor of LEF/TCF target genes and promotes myogenic differentiation by interacting with beta-catenin. Monoclonal antibody F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. The F4B2-B11 antibody is cross reactive with mouse and rat FHL2.
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha
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