Mature Macrophages antibody

Catalog No. ABIN2192020

This Mouse Monoclonal antibody specifically detects Mature Macrophages in IHC (fro), FACS, IHC (p) and WB. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for Mature Macrophages antibody (ABIN2192020)

Target: Mature Macrophages

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: 25F9

Product Details

Sterility: 0.2 μm filtered

Application Details

Application Notes: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Rosseau, Hammerl, Maus, Walmrath, Schütte, Grimminger, Seeger, Lohmeyer: "Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 279, Issue 1, pp. L25-35, (2000) (PubMed).

Kiefer, Kieseier, Brück, Hartung, Toyka: "Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies." in: Brain : a journal of neurology, Vol. 121 ( Pt 3), pp. 469-79, (1998) (PubMed).

Zwadlo, Bröcker, von Bassewitz, Feige, Sorg: "A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 134, Issue 3, pp. 1487-92, (1985) (PubMed).

Target Details

Target: Mature Macrophages

Background: The monoclonal antibody 25F9 recognises a protein of 86 kD on the cell surface and within the cytoplasm of mature macrophages. The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. Furthermore the monoclonal antibody 25F9 cross reacts with a subpopulation of macrophages of rhesus monkey, pig alveolar macrophages and Kupffer cells. The monoclonal antibody 25F9 is very useful for macrophage phenotyping.