Activated Cd11/cd18 antibody

Catalog No. ABIN2192042

Product Details

Target: Activated Cd11/cd18

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunoprecipitation (IP), Functional Studies (Func), Immunoassay (IA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Sterility: 0.2 μm filtered

Clone: 24

Application Details

Application Notes: For immunohistology and flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts activated CD11/CD18 to be inhibited.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Hogg, Laschinger, Giles, McDowall: "T-cell integrins: more than just sticking points." in: Journal of cell science, Vol. 116, Issue Pt 23, pp. 4695-705, (2003) (PubMed).

Kamata, Tieu, Tarui, Puzon-McLaughlin, Hogg, Takada: "The role of the CPNKEKEC sequence in the beta(2) subunit I domain in regulation of integrin alpha(L)beta(2) (LFA-1)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 5, pp. 2296-301, (2002) (PubMed).

Dransfield, Cabañas, Barrett, Hogg: "Interaction of leukocyte integrins with ligand is necessary but not sufficient for function." in: The Journal of cell biology, Vol. 116, Issue 6, pp. 1527-35, (1992) (PubMed).

Dransfield, Hogg: "Regulated expression of Mg2+ binding epitope on leukocyte integrin alpha subunits." in: The EMBO journal, Vol. 8, Issue 12, pp. 3759-65, (1990) (PubMed).

Hogg, Selvendran: "An anti-human monocyte/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue." in: Cellular immunology, Vol. 92, Issue 2, pp. 247-53, (1985) (PubMed).

Target Details

Target: Activated Cd11/cd18

Background: The monoclonal antibody 24 recognizes an epitope of 174 kD present on leukocyte integrins, CD11/CD18 or beta 2-type integrins. The leukocyte integrins are a family of heterodimeric receptors that mediate divalent cation-dependent cellular adhesion reactions. T cells use integrins in essentially all of their functions. Integrins become active following signalling through other membrane receptors, which cause both affinity alteration and an increase in integrin clustering. The monoclonal antibody 24 recognizes a structural feature and is strictly dependent upon the presence of Mg 2+. The epitope is located within, or in close proximity to, the three conserved cation binding domains and therefore a measure of Mg 2+ bound to the leukocyte integrins and thus reflects functionally active molecules. The epitope can be induced on polymorphonuclear leukocytes and on monocytes. Glu173 and Glu175 of the beta(2) I domain are identified as critical for antibody 24 binding. The monoclonal antibody 24 inhibits monocyte-dependent, antigen specific T cell proliferation and IL-2- activated natural killer cell assays. The antibody does not interfere with mitogen-stimulated T cell proliferation. Furthermore the monoclonal antibody 24 prevents "deadhesion" of receptor/ligand pairs, possible locking leukocyte integrins in an "active" conformation.