Leptin Receptor antibody (Soluble)

Catalog No. ABIN2192046

This anti-Leptin Receptor antibody is a Mouse Monoclonal antibody detecting Leptin Receptor in WB, IHC (p), IHC (fro) and IA. Suitable for Human. This Primary Antibody has been cited in 1 publication.

Quick Overview for Leptin Receptor antibody (Soluble) (ABIN2192046)

Target: Leptin Receptor (LEPR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Leptin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)

Clone: 2F1

Product Details anti-Leptin Receptor Antibody

Binding Specificity: Soluble

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blot, immuno assays and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Leptin Receptor antibody (ABIN2192046)

van Dielen, van t Veer, Buurman, Greve: "Leptin and soluble leptin receptor levels in obese and weight-losing individuals." in: The Journal of clinical endocrinology and metabolism, Vol. 87, Issue 4, pp. 1708-16, (2002) (PubMed).

Target Details for Leptin Receptor

Target: Leptin Receptor (LEPR)

Background: The monoclonal antibody 2F1 reacts with human soluble leptin receptor (sLR) in plasma. Leptin is a cytokine that is primarily expressed by adipose tissue. Leptin controls food intake by its interaction with the leptin receptor in the brain. Leptin action is mediated and controlled by the leptin receptor, a class I type cytokine receptor. sLR is generated by proteolytic cleavage of membrane-anchored receptors. This indicates that the leptin receptor might have other functions besides transduction. Binding of leptin with sLR increases the bioavailability of leptin in plasma, but also decreases the binding of leptin to membrane bound leptin receptors. For example, when comparing obese and lean individuals, plasma levels of sLR are significantly decreased whereas leptin levels are significantly increased. The monoclonal antibody 2F1 specifically reacts with sLR with a molecular mass of 180kD. The 2F1 antibody can be used to measure in both free sLR and sLR bound to leptin in plasma.

Pathways: JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour