SFTPD antibody

Catalog No. ABIN2192111

Product Details anti-SFTPD Antibody

Target: SFTPD (Surfactant Protein D (SFTPD))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SFTPD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: IIIH3

Application Details

Application Notes: For immunohistology and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-SFTPD antibody (ABIN2192111)

ter Horst, Fijlstra, Sengupta, Walther, Wagenaar: "Spatial and temporal expression of surfactant proteins in hyperoxia-induced neonatal rat lung injury." in: BMC pulmonary medicine, Vol. 6, pp. 8, (2006) (PubMed).

Kasper, Albrecht, Grossmann, Grosser, Schuh, Müller: "Monoclonal antibodies to surfactant protein D: evaluation of immunoreactivity in normal rat lung and in a radiation-induced fibrosis model." in: Experimental lung research, Vol. 21, Issue 4, pp. 577-88, (1995) (PubMed).

Target Details for SFTPD

Target: SFTPD (Surfactant Protein D (SFTPD))

Alternative Name: Surfactant Protein D (SFTPD Products)

Synonyms: COLEC7 antibody, PSP-D antibody, SFTP4 antibody, SP-D antibody, BDE antibody, BDSD antibody, HOX4I antibody, SPD antibody, AI573415 antibody, Sftp4 antibody, surfactant protein D antibody, homeobox D13 antibody, surfactant associated protein D antibody, SFTPD antibody, HOXD13 antibody, Sftpd antibody, SP-D antibody

Background: The monoclonal antibody IIIH3 recognizes the rat surfactant protein D (SP-D). SP-D belongs to the collectin familiy. These proteins are oligomeric proteins composed of carbohydrate-recognition domains (CRD) attached to collagenous regions. They are structurally similar to the ficolins although they make use of different CRD structures: C-type lectin domain for the collectins. The anti-microbial effector mechanisms of SP-D are direct opsonization, neutralization, and agglutination. Thus limiting the infection and concurrently orchestrating the subsequent adaptive immune response. The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type II cells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointeststinal tract and in epithelial cells of exocrine glands. SP-D synthesis and secretion increase significantly after inflammatory stress. Increased amounts of SP-D in lavage and tissue, particularly in type II pneumocytes, in Clara cells and in hyperplastic goblet cells are found in inflamed lungs. The localization of SP-D in endocytic vesicles and in lysosomal granules of alveolar macrophages suggests that a receptor-mediated uptake occurs. SP-D binds to apoptotic neutrophils and enhances their clearance by alveolar macrophages. Monoclonal antibody IIIH3 specific for rat surfactant protein D shows significant cross reactivity with human SP-D.