SCARB1 antibody

Catalog No. ABIN2192115

This anti-SCARB1 antibody is a Mouse Monoclonal antibody detecting SCARB1 in FACS, IHC (fro) and Neut. Suitable for Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for SCARB1 antibody (ABIN2192115)

Target: SCARB1 (Scavenger Receptor Class B, Member 1 (SCARB1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SCARB1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Neutralization (Neut)

Clone: 3D12

Product Details anti-SCARB1 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For flow cytometry and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For neutralization of biological activity in vitro dilutions have to be made according to the amounts of SR-BI to be inactivated.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-SCARB1 antibody (ABIN2192115)

Nakagawa, Nagaosa, Hirose, Tsuda, Hasegawa, Shiratsuchi, Nakanishi: "Expression and function of class B scavenger receptor type I on both apical and basolateral sides of the plasma membrane of polarized testicular Sertoli cells of the rat." in: Development, growth & differentiation, Vol. 46, Issue 3, pp. 283-98, (2004) (PubMed).

Target Details for SCARB1

Target: SCARB1 (Scavenger Receptor Class B, Member 1 (SCARB1))

Alternative Name: Scavenger Receptor Bi

Background: Monoclonal antibody 3D12 reacts with rat class B scavenger receptor type I (SR-BI). Scavenger receptors have been studied primarily for their ability to bind and internalize modified lipoproteins. They have been found in the development of atherosclerosis and other macrophage-associated functions. Scavenger receptors also function as pattern recognition receptors for a wide variety of pathogens. This finding indicates a potential role in host defense. SR-BI belongs together with CD36 to the class B scavenger receptor family. SR-BI is a multiligand membrane protein existing in various organs such as the liver and various cell types such as endothelial cells, macrophages, brain cells, Leydig cells and Sertoli cells. SR-BI has been found as a receptor for phospholipids, free and (lipo)protein-bound ApoE, lipid-bound ApoA-I, HDL, hypochlorite- modified LDL and more. In liver, the PDZK-1 (and possible other PDZ domains) of SR-BI has been found to be essential for cell surface expression and, hence, reverse cholesterol transport. In the brain, the presence of SR-BI seems to be involved in the uptake of oxidatively modified lipoproteins and beta- amyloid protein complexed with ApoE, suggesting SR-BI to be an important tool for studies on neurodegenerative disorders. In the testis, SR-BI is expressed in two somatic cell types: Leydig cells and Sertoli cells. SR-BI functions at least partly as a phosphatidyl serine receptor (PSR), enabling Sertoli cells to recognize and phagocytose apoptotic spermatogenic cells at all stages of differentiation. Monoclonal antibody 3D12 blocks the biological activity of rat SR-BI. For example, it inhibits the ability of SR-BI to mediate the corporation of lipids of HDL by SR-BI expressing cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Lipid Metabolism, SARS-CoV-2 Protein Interactome