C3 antibody

Catalog No. ABIN2192120

The Mouse Monoclonal anti-C3 antibody has been validated for WB and IA. It is suitable to detect C3 in samples from Rat.

Quick Overview for C3 antibody (ABIN2192120)

Target: C3 (Complement Component 3 (C3))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This C3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoassay (IA)

Clone: 2B10B9B2

Product Details anti-C3 Antibody

Endotoxin Level: Low endotoxin level

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: 1 mL(> 100 μg/mL) culture medium with a low endotoxin level containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details for C3

Target: C3 (Complement Component 3 (C3))

Alternative Name: c3/c3b

Background: The monoclonal antibody 2B10B9B2 reacts with an epitope on C3b. The antibody reacts both with intact C3 and C3b. The complement factor C3 consists of an alpha- and a beta-chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical and mannose binding lectin activation pathways lead to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to formation of the alpha- chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues including renal epithelial cells and hepatocytes.

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling