Shiga Toxin 2 (Stx-2) antibody

Catalog No. ABIN2192154

Cited in 2+ publications. The Mouse Monoclonal anti-Shiga Toxin 2 antibody (Clone 11E10) (ABIN2192154) specifically detects Shiga Toxin 2 in IA, InhA and WB. The antibody is reactive with Shigella dysenteriae samples.

Quick Overview for Shiga Toxin 2 (Stx-2) antibody (ABIN2192154)

Target: Shiga Toxin 2 (Stx-2)

Reactivity: Shigella dysenteriae

Host: Mouse

Clonality: Monoclonal

Application: Immunoassay (IA), Inhibition Assay (InhA), Western Blotting (WB)

Clone: 11E10

Product Details

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of toxin to be inactivated.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Smith, Teel, Carvalho, Melton-Celsa, OBrien: "Development of a hybrid Shiga holotoxoid vaccine to elicit heterologous protection against Shiga toxins types 1 and 2." in: Vaccine, Vol. 24, Issue 19, pp. 4122-9, (2006) (PubMed).

Perera, Marques, OBrien: "Isolation and characterization of monoclonal antibodies to Shiga-like toxin II of enterohemorrhagic Escherichia coli and use of the monoclonal antibodies in a colony enzyme-linked immunosorbent assay." in: Journal of clinical microbiology, Vol. 26, Issue 10, pp. 2127-31, (1988) (PubMed).

Target Details

Target: Shiga Toxin 2 (Stx-2)

Background: The monoclonal antibody 11E10 recognizes the 2A subunit of Shiga-like toxin 2. Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11, and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share >99 % deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60 % deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. Antibodies to SLT-II can also neutralize a variant of SLT-II (designated SLT-Iiv) produced by strains of E.coli that cause edema. SLT-IIv is cytotoxic for Vero but not HeLa cells, distinguishing it from SLT-II. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.