CRISP3 antibody

Catalog No. ABIN2192192

The Rabbit Polyclonal anti-CRISP3 antibody has been validated for WB, IHC (p) and IA. It is suitable to detect CRISP3 in samples from Human. There are 3+ publications available.

Quick Overview for CRISP3 antibody (ABIN2192192)

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRISP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoassay (IA)

Product Details anti-CRISP3 Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For Western blotting and immunohistology dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-CRISP3 antibody (ABIN2192192)

Udby, Cowland, Johnsen, Sørensen, Borregaard, Kjeldsen: "An ELISA for SGP28/CRISP-3, a cysteine-rich secretory protein in human neutrophils, plasma, and exocrine secretions." in: Journal of immunological methods, Vol. 263, Issue 1-2, pp. 43-55, (2002) (PubMed).

Udby, Calafat, Sørensen, Borregaard, Kjeldsen: "Identification of human cysteine-rich secretory protein 3 (CRISP-3) as a matrix protein in a subset of peroxidase-negative granules of neutrophils and in the granules of eosinophils." in: Journal of leukocyte biology, Vol. 72, Issue 3, pp. 462-9, (2002) (PubMed).

Kjeldsen, Cowland, Johnsen, Borregaard: "SGP28, a novel matrix glycoprotein in specific granules of human neutrophils with similarity to a human testis-specific gene product and a rodent sperm-coating glycoprotein." in: FEBS letters, Vol. 380, Issue 3, pp. 246-50, (1996) (PubMed).

Target Details for CRISP3

Target: CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))

Alternative Name: Crisp-3

Background: Cysteine-rich secretory protein 3 (CRISP-3, also known as SGP28) is a glycoprotein that belongs to the family of cysteine-rich secretory proteins (CRISPs) which was originally discovered in human neutrophilic granulocytes. CRISP-3 is also widely distibuted in exocrine glands (salivary glands, pancreas and prostate), eosinophilic granulocytes and to a lower level in epididymis, ovary, thymus and colon. The presence of CRISP-3 in neutrophils, eosinophils and in exocrine secretions indicates a role in innate host defense. The antibody has been raised against recombinant C-terminally truncated form of CRISP-3 and recognizes both the N-glycosylated and non-glycosylated form of the mature protein.