Caspase 3 antibody (N-Term)

Catalog No. ABIN219227

The Rabbit Polyclonal anti-Caspase 3 antibody has been validated for WB, IHC, ELISA, IP and IHC (p). It is suitable to detect Caspase 3 in samples from Human, Mouse and Rat.

Quick Overview for Caspase 3 antibody (N-Term) (ABIN219227)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 3 Antibody

Binding Specificity: N-Term

Specificity: Recognizes human caspase-3 (35kD) and the large fragment of caspase-3 resulting from cleavage (17, 19kD). Species cross-reactivity: mouse and rat.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3 (KLH).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:200. Immunoprecipitation: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation