Caspase 3 antibody (N-Term)

Catalog No. ABIN219240

This anti-Caspase 3 antibody is a Rabbit Polyclonal antibody detecting Caspase 3 in WB. Suitable for Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Guinea Pig, Sheep, Chicken and Saccharomyces cerevisiae.

Quick Overview for Caspase 3 antibody (N-Term) (ABIN219240)

Target: Caspase 3 (CASP3)

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Pig, Guinea Pig, Sheep, Chicken, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 3 Antibody

Binding Specificity: N-Term

Specificity: Recognizes human caspase-3. In apoptotic cells, this antibody also detects 20kD and 18kD proteins which correspond to the processed forms of caspase-3. Following the induction of apoptosis and the appearance of these smaller bands, a concurrent reduction in the amount of the procaspase enzyme is noted on immunoblot analysis. A weak ~30kD band may be detected in some samples. This band is not caspase-3 as it is detected in MCF7 cell lysates which lack the caspase-3 protein. Species cross-reactivity: mouse, rat, bovine, canine, chicken, guinea pig, hamster, monkey, porcine, rabbit and yeast.

Purification: Protein A purified

Immunogen: Recombinant full length human caspase-3 protein with an N-terminal (His) 6 tag.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Suitable for use in Western Blot. Western Blot (ECL): 1 μg/mL. A band is seen at ~36kD. Positive control: HeLa Nuclear Extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: CASP3 / Caspase 3

Background: Name/Gene ID: CASP3
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP3, Apopain, CASP-3, Caspase-3, CPP32B, CPP32, Cysteine protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Procaspase3, Caspase 3, CPP-32, PARP cleavage protease, Protein Yama

Gene ID: 836

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation