GAD65+GAD67 (C-Term) antibody

Catalog No. ABIN220265

The Rabbit Polyclonal anti-GAD65+GAD67 antibody is suitable to detect GAD65+GAD67 in samples from Mouse, Human, Rat, Cow, Dog, Pig, Bat, Hamster, Horse, Monkey, Rabbit, Xenopus laevis and Zebrafish (Danio rerio). It has been validated for WB, IHC, ELISA and IHC (fro).

Quick Overview for GAD65+GAD67 (C-Term) antibody (ABIN220265)

Target: GAD65+GAD67

Reactivity: Mouse, Human, Rat, Cow, Dog, Pig, Bat, Hamster, Horse, Monkey, Rabbit, Xenopus laevis, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details

Binding Specificity: C-Term

Specificity: Recognizes both molecular forms of glutamic acid decarboxylase (GAD65 and GAD67), the enzyme which converts glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region. Species cross-reactivity: human, rat and feline.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Cat, Dog, Horse, Rabbit, Pig, Opossum, Platypus, Lizard, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%) Turkey, Chicken (93%).

Purification: Ammonium sulfate precipitation

Immunogen: Synthetic peptide sequence DFLIEEIERLGQDL from the C-terminus of GAD. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Cat, Dog, Horse, Rabbit, Pig, Opossum, Platypus, Lizard, Xenopus, Seabass, Stickleback, Pufferfish, Zebrafish (100%), Turkey, Chicken (93%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, WB (1:1000 - 1:10000)

Usage: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Immunohistochemistry (Frozen): 1:200-1:5000. Western Blot: 1:1000-1:10000. Positive control: Brain.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: GAD65+GAD67

Alternative Name: GAD 65+67

Background: This antibody detects: GAD 65+67

UniProt: Q05329