GAD65+GAD67 (AA 520-600) antibody

Catalog No. ABIN7214988

The Rabbit Polyclonal anti-GAD65+GAD67 antibody (ABIN7214988) specifically detects GAD65+GAD67 in WB, IHC, ELISA and IF. The antibody is reactive with Mouse and Human samples.

Quick Overview for GAD65+GAD67 (AA 520-600) antibody (ABIN7214988)

Target: GAD65+GAD67

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details

Binding Specificity: AA 520-600

Purpose: Rabbit Anti-GAD-65/67 Polyclonal Antibody

Specificity: GAD-65/67 Polyclonal Antibody detects endogenous levels of GAD-65/67 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human GAD-65/67 at AA range: 520-600

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: GAD65+GAD67

Alternative Name: GAD-65/67

Background: GAD1, GAD, GAD67, Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform, GAD2, GAD65, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65GAD1 encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. Glutamate decarboxylase 1 is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Molecular Weight: 65kD

Gene ID: 2571, 2572

UniProt: Q99259, Q05329