HSP27 antibody (pSer82)

Catalog No. ABIN220305

This anti-HSP27 antibody is a Rabbit Polyclonal antibody detecting HSP27 in WB, IHC, IHC (p) and ICC. Suitable for Human and Monkey.

Quick Overview for HSP27 antibody (pSer82) (ABIN220305)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-HSP27 Antibody

Binding Specificity: pSer82

Specificity: Detects endogenous human HSP27 only when phosphorylated at serine 82. Does not recognize other heat shock proteins. Species cross-reactivity: Monkey.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser82 of human HSP27.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry (ABC). Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % BSA, 1XTBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (ABC): 1:500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 / HSP27

Background: Name/Gene ID: HSPB1

Synonyms: HSPB1, 28 kDa heat shock protein, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27  kDa protein 1, HSP27, HSP28, HSP 27, Hsp25, SRP27, Stress-responsive protein 27, CMT2F, Heat shock 27 kd protein, Heat shock protein beta-1, HMN2B, HS.76067

Gene ID: 3315

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling