ERK2 antibody (pThr180, Tyr182)

Catalog No. ABIN220473

This anti-ERK2 antibody is a Rabbit Polyclonal antibody detecting ERK2 in WB, ELISA, IF, IP, ICC and FACS. Suitable for Human.

Quick Overview for ERK2 antibody (pThr180, Tyr182) (ABIN220473)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-ERK2 Antibody

Binding Specificity: pThr180, Tyr182

Specificity: Detects endogenous levels of p38 MAP kinase only when activated by dual phosphorylation at Thr180 and Tyr182. It will also react with p38 singly phosphorylated at Thr180 and singly phosphorylated at Tyr182. Does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species cross-reactivity: Human.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues around Thr180/Tyr182 of human p38 MAP kinase.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:100), ICC (1:100), IF, IP (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Immunoprecipitation: 1:50. Immunofluorescence/ Immunocytochemistry: 1:100. Flow Cytometry: 1:100.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM sodium HEPES,  pH 7.5, 150 mM sodium chloride, BSA, 50 % glycerol.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: MAPK1 / ERK2 / p42

Background: Name/Gene ID: MAPK1
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2

Gene ID: 5594

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins