PARP1 antibody (N-Term)

Catalog No. ABIN220760

The Rabbit Polyclonal anti-PARP1 antibody has been validated for WB and IHC. It is suitable to detect PARP1 in samples from Human and Cow.

Quick Overview for PARP1 antibody (N-Term) (ABIN220760)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PARP1 Antibody

Binding Specificity: N-Term

Specificity: Specifically recognizes the 85kD fragment of cleaved PARP and can be used as marker for detecting apoptotic cells. Species cross-reactivity: Human and bovine. Mouse and rat demonstrate 86 % homology.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to N-terminus of cleavage site (214/215) of human PARP.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, WB (1:1000)

Usage: Suitable for use in Western Blot, Immunohistochemistry. Western Blot: 1:1000. Positive control: HeLa and Jurkat cells treated with staurosporine.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Dulbecco's PBS (without Mg2+, Ca2+),  pH 7.3, 50 % glycerol, 1 mg/mL BSA (IgG, protease free), 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 / PARP

Background: Name/Gene ID: PARP1

Synonyms: PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location