PARP1 antibody

Catalog No. ABIN220764

The Rabbit Polyclonal anti-PARP1 antibody (ABIN220764) specifically detects PARP1 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PARP1 antibody (ABIN220764)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PARP1 Antibody

Specificity: Detects endogenous levels of full length PARP (116kD), as well as the large fragment (89kD) and small fragment (24kD) of PARP resulting from caspase cleavage. Does not cross-react with related proteins.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (KLH coupled) corresponding to the caspase cleavage site in PARP.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB (1:1000)

Usage: Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 / PARP

Background: Name/Gene ID: PARP1

Synonyms: PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location