PARP1 antibody (C-Term)

Catalog No. ABIN220765

This Rabbit Polyclonal antibody specifically detects PARP1 in WB, ELISA, IHC, IF, FACS, ICC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PARP1 antibody (C-Term) (ABIN220765)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PARP1 Antibody

Binding Specificity: C-Term

Specificity: Detects endogenous levels of the large fragment (89kD) of human PARP produced by caspase cleavage. Does not react with full length PARP.

Purification: Protein A purified

Immunogen: Synthetic peptide (KLH coupled) corresponding to C-terminal residues adjacent to Asp214 in human PARP.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:25), ICC, IF, IHC, IHC-P (1:50), WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:50. Immunocytochemistry (IF): 1:200. Flow Cytometry: 1:25.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP1 / PARP

Background: Name/Gene ID: PARP1

Synonyms: PARP1, ADPRT, ADP-ribosyltransferase NAD(+), Adp-ribosyltransferase, ADPRT1, ARTD1, ADPRT 1, Poly(ADP-ribose) synthetase, Poly(ADP-ribosyl)transferase, PARP, Poly [ADP-ribose] polymerase 1, PADPRT-1, PARP-1, Poly (ADP-ribose) polymerase 1, Poly(ADP-ribose) polymerase, Poly[ADP-ribose] synthase 1, PPOL

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location