JNK antibody (pThr183, Tyr185)

Catalog No. ABIN220912

This Mouse Monoclonal antibody specifically detects JNK in WB, ELISA, IP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for JNK antibody (pThr183, Tyr185) (ABIN220912)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-JNK Antibody

Binding Specificity: pThr183, Tyr185

Specificity: Detects endogenous levels of human p46 and p54 SAPK/JNK dually phosphorylated at threonine 183 and tyrosine 185. Does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase. Species cross-reactivity: Human, mouse and rat.

Purification: Immunoaffinity purified

Immunogen: Synthetic phosphopeptide corresponding to residues around Thr183/Tyr185 of human SAPK/JNK (KLH coupled).

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1:200), IP (1:250), WB (1:2000)

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Flow Cytometry. Immunoprecipitation: 1:250. Flow Cytometry: 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Short term: -20°C
Long term: -20°C.

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8 / JNK1 / JNK

Background: Name/Gene ID: MAPK8
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK8, JNK, JNK1, JNK1A2, JNK-46, MAP kinase 8, PRKM8, SAPK1, SAPK1c, MAPK 8, C-Jun N-terminal kinase 1, JNK21B1/2, JUN N-terminal kinase

Gene ID: 5599

UniProt: P45983

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins